Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry
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چکیده
منابع مشابه
Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry.
Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC were compared with visual scoring results obtained from slides of cells stained using Fast Green and 4',6-diamidino-2-phenylindole (DAPI). This fl...
متن کاملThe imaging flow cytometry cytokinesis‐block micronucleus assay for radiation biodosimetry
The cytokinesis-block micronucleus (CBMN) assay is an accepted method for biodosimetry where the dose of radiation to potentially exposed individuals is estimated from the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay is traditionally performed using a microscope-based scoring procedure which can be labour intensive, time consuming and subject to variability of...
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In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimeti...
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The cytokinesis-block micronucleus (CBMN) assay is a well-established technique that can be employed in triage radiation biodosimetry to estimate whole body doses of radiation to potentially exposed individuals through quantitation of the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay has been partially automated using traditional microscope-based methods and mo...
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Background: Most of the cytogenetic dosimetry, except Premature Chromosome Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB-MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0-15Gy, cultured for 72 h. Cytoch...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2014
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114239